The Effects of Madol on the Physical Activity Research Paper

The Effects of Madol on the Physical Activity - Research study ExampleFor the purpose of this study 3-month-old adult Hamsters (Mesocricetus auratus) entrust be reared in one climate-controlled room in standard plastic cages. Standard food pellets and water will be provided ad libitum. The light/ distressing conditions will be 1212 with the light intensity varying between 200 to three hundred Lx (Unit of luminance) during the day and 5 Lx (Unit of luminance) during the dark period. The temperature will be regulated to 25 + 2 Celsius and the relative humidity will be maintained between 60 to 65 %. The protocols for using the hamsters for the experiments will be reviewed and approved by an Institutional Animal Care and Use Committee (IACUC) and all matter Institute of Health (NIH) guidelines and procedures for care and use of laboratory animals will be followed. Hamsters (n = 80) will be shared into four groups. Group I (n = 8) will receive an intraperitoneal injection of an equi valent volume of ethanol employ in other groups for drug delivery. Group II (n = 24, 8 per each dose) will be injected subcutaneously with testosterone (1,2 or 5 mg/Kg body weight). Group III (n = 24, 8 per each dose) will be injected intraperitoneally with dihydrotestosterone (DHT, 1,2, or 5mg/Kg body weight). Group IV (n = 24, 8 per each dose) will be treated with major (DMT, 1,2, or 5 mg/Kg body weight) intraperitoneally. Testosterone, DHT (derived from testosterone by enzyme 5-alpha reductase) and DMT will be dissolved in ethanol. Testosterone and DHT are natural anabolic androgenic steroids present in the human body. dosage levels are derived from the extensive literature review for testosterone and DHT. All these drugs will be procured from a chemical company.